Efek Neuroprotektif Fraksi Air Daun Semanggi (Marsilea crenata Presl.) secara In Vitro

Background: Estrogen deficiency can trigger several diseases, one of which is neurodegenerative. Neurodegenerative begins with neuroinflammation, triggers the activation microglia cells resulting in a pro-inflammatory activity. Potential and relatively safe therapy to use overcome it by using phytoestrogen compounds. Marsilea crenata Presl. plant that contains phytoestrogens. Objective: The purpose this study was analyze neuroprotective effect water fraction leaves shown inhibition neuroinflammation marked increased levels MHC II against HMC3 cells. Methods: IFN-γ induced into for 24 hours cause inflammatory conditions. given at dose 62,5; 125; 250 µg / ml. Analysis ICC method aid CLSM instrument. Results: results indicate reduce expression concentrations 125 μg ml values ​​of 465,748 460,884 AU p<0,005. Conclusion: This concludes has activity doses μg/ml IFN-γ. ED50 value 0,582 μg/ml.